A Kinetic and Microautoradiographic Analysis of ['4C]Sucrose lmport by Developing Wheat Crains'

A Kinetic and Microautoradiographic Analysis of [14C]Sucrose Import by Developing Wheat Grains.

Assimilates enter developing wheat grains via a strand of phloem extending along the crease region of the grain. After phloem unloading, they move several hundred micrometers before being released into the endosperm cavity, from which they are absorbed by the developing endosperm. Extraphloem assimilate pools in the maternal tissue of the crease, therefore, play a central role in post-phloem tr...

Developmental Regulation sof the Plastid Protein lmport Apparatus

Plastid development involves the programmed accumulation of proteins. Most plastid proteins are synthesized in the cytosol and imported into the organelle by an envelope-based protein import apparatus. Previous studies have shown that developmental rates of protein accumylation correspond to mRNA levels. Here, we examined the relationship between plastid development and the activity of the prot...

Properties of Pyrophosphate : Fructose - 6 - Phosphate Phosphotransferase from Endosperm of Developing Wheat ( Triticum aestivum L . ) Grains 1

Pyrophosphate:fructose-6-phosphate phosphotransferase (PFP, EC 2.7.1.90) from endosperm of developing wheat (Triticum aestivum L.) grains was purified to apparent homogeneity with about 52% recovery using ammonium sulfate fractionation, ion exchange chromatography on DEAE-cellulose and gel filtration through Sepharose-CL-6B. The purified enzyme, having a molecular weight of about 170,000, was a...

Application of a Kinetic Model for Studying Impurities Effect on Crystallization of NaCl, KBr, ADP and Sucrose Solutions

Characterization of wheat germ protein synthesis initiation factor eIF-4C and comparison of eIF-4C from wheat germ and rabbit reticulocytes.

Eukaryotic protein synthesis initiation factor (eIF)-4C was purified from wheat germ and the molecular weight was calculated to be approximately 19,000 by SDS-polyacrylamide gel electrophoresis. A similar molecular weight was determined by gel filtration chromatography indicating that wheat germ eIF-4C is functional as a single polypeptide chain. An efficient in vitro translation system depende...